Articles, books and book chapters

@article{dezetter_behavioural_2022,

title = {Behavioural hydroregulation protects against acute effects of drought in a dry-skinned ectotherm},

author = {Mathias Dezetter and Jean-François Le Galliard and Olivier Lourdais},

url = {https://doi.org/10.1007/s00442-022-05299-1},

doi = {10.1007/s00442-022-05299-1},

issn = {1432-1939},

year  = {2022},

date = {2022-12-01},

urldate = {2023-01-06},

journal = {Oecologia},

abstract = {During extreme climate events, behavioural thermoregulation may buffer ectotherms from thermal stress and overheating. However, heatwaves are also combined with dry spells and limited water availability, and how much individuals can behaviourally mitigate dehydration risks through microclimate selection remains largely unknown. Herein, we investigated the behavioural and physiological responses to changes in air and microhabitat humidity in a terrestrial ectotherm, the asp viper (Vipera aspis). We exposed individuals to a simulated heatwave together with water deprivation for 3 weeks, and manipulated air water vapour density (wet air vs. dry air) and microclimate (wet shelter vs. dry shelter) in a two-by-two factorial design. Dry air conditions led to substantial physiological dehydration and muscle wasting. Vipers exposed to dry air used more often a shelter that offered a moist microclimate, which reduced dehydration and muscle wasting at the individual level. These results provide the first experimental evidence that active behavioural hydroregulation can mitigate specific physiological stress responses caused by a dry spell in an ectotherm. Future studies investigating organismal responses to climate change should consider moisture gradient in the habitat and integrate both hydroregulation and thermoregulation behaviours.},

keywords = {behaviour, Humidity gradient, hydroregulation, Microclimate selection, Water},

pubstate = {published},

tppubtype = {article}

}

@article{chabaud_climate_2022,

title = {Climate aridity and habitat drive geographical variation in morphology and thermo-hydroregulation strategies of a widespread lizard species},

author = {Chloé Chabaud and Matthieu Berroneau and Maud Berroneau and Andréaz Dupoué and Michaël Guillon and Robin Viton and Rodrigo S B Gavira and Jean Clobert and Olivier Lourdais and Jean-François Le Galliard},

url = {https://doi.org/10.1093/biolinnean/blac114},

doi = {10.1093/biolinnean/blac114},

issn = {0024-4066},

year  = {2022},

date = {2022-10-01},

urldate = {2022-10-18},

journal = {Biological Journal of the Linnean Society},

pages = {blac114},

abstract = {Thermo-hydroregulation strategies involve concurrent changes in functional traits related to energy, water balance and thermoregulation and play a key role in determining life-history traits and population demography of terrestrial ectotherms. Local thermal and hydric conditions should be important drivers of the geographical variation of thermo-hydroregulation strategies, but we lack studies that examine these changes across climatic gradients in different habitat types. Here, we investigated intraspecific variation of morphology and thermo-hydroregulation traits in the widespread European common lizard (Zootoca vivipara louislantzi) across a multidimensional environmental gradient involving independent variation in air temperature and rainfall and differences in habitat features (access to free-standing water and forest cover). We sampled adult males for morphology, resting metabolic rate, total and cutaneous evaporative water loss and thermal preferences in 15 populations from the rear to the leading edge of the distribution across an elevational gradient ranging from sea level to 1750 m. Besides a decrease in adult body size with increasing environmental temperatures, we found little effect of thermal conditions on thermo-hydroregulation strategies. In particular, relict lowland populations from the warm rear edge showed no specific ecophysiological adaptations. Instead, body mass, body condition and resting metabolic rate were positively associated with a rainfall gradient, while forest cover and water access in the habitat throughout the season also influenced cutaneous evaporative water loss. Our study emphasizes the importance of rainfall and habitat features rather than thermal conditions for geographical variation in lizard morphology and physiology.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{dupoue_lizards_2022,

title = {Lizards from warm and declining populations are born with extremely short telomeres},

author = {Andréaz Dupoué and Pauline Blaimont and Frédéric Angelier and Cécile Ribout and David Rozen-Rechels and Murielle Richard and Donald Miles and Pierre Villemereuil and Alexis Rutschmann and Arnaud Badiane and Fabien Aubret and Olivier Lourdais and Sandrine Meylan and Julien Cote and Jean Clobert and Jean-François Le Galliard},

url = {https://www.pnas.org/doi/abs/10.1073/pnas.2201371119},

doi = {10.1073/pnas.2201371119},

year  = {2022},

date = {2022-08-01},

urldate = {2022-08-16},

journal = {Proceedings of the National Academy of Sciences},

volume = {119},

number = {33},

pages = {e2201371119},

note = {Publisher: Proceedings of the National Academy of Sciences},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{voituron_mitochondrial_2022,

title = {Mitochondrial oxidative phosphorylation response overrides glucocorticoid-induced stress in a reptile},

author = {Yann Voituron and Damien Roussel and Jean-François Le Galliard and Andréaz Dupoué and Caroline Romestaing and Sandrine Meylan},

url = {https://doi.org/10.1007/s00360-022-01454-5},

doi = {10.1007/s00360-022-01454-5},

issn = {1432-136X},

year  = {2022},

date = {2022-08-01},

urldate = {2022-08-16},

journal = {Journal of Comparative Physiology B},

abstract = {Stress hormones and their impacts on whole organism metabolic rates are usually considered as appropriate proxies for animal energy budget that is the foundation of numerous concepts and models aiming at predicting individual and population responses to environmental stress. However, the dynamics of energy re-allocation under stress make the link between metabolism and corticosterone complex and still unclear. Using ectopic application of corticosterone for 3, 11 and 21 days, we estimated a time effect of stress in a lizard (Zootoca vivipara). We then investigated whole organism metabolism, muscle cellular O2 consumption and liver mitochondrial oxidative phosphorylation processes (O2 consumption and ATP production) and ROS production. The data showed that while skeletal muscle is not impacted, stress regulates the liver mitochondrial functionality in a time-dependent manner with opposing pictures between the different time expositions to corticosterone. While 3 days exposition is characterized by lower ATP synthesis rate and high H2O2 release with no change in the rate of oxygen consumption, the 11 days exposition reduced all three fluxes of about 50%. Oxidative phosphorylation capacities in liver mitochondria of lizard treated with corticosterone for 21 days was similar to the hepatic mitochondrial capacities in lizards that received no corticosterone treatment but with 40% decrease in H2O2 production. This new mitochondrial functioning allows a better capacity to respond to the energetic demands imposed by the environment but do not influence whole organism metabolism. In conclusion, global mitochondrial functioning has to be considered to better understand the proximal causes of the energy budget under stressful periods.},

keywords = {corticosterone, lizard, metabolism, Mitochondrial efficiency, Oxygen consumption, ROS and ATP production},

pubstate = {published},

tppubtype = {article}

}

@article{reinke_diverse_2022,

title = {Diverse aging rates in ectothermic tetrapods provide insights for the evolution of aging and longevity},

author = {Beth A. Reinke and Hugo Cayuela and Fredric J. Janzen and Jean-François Lemaître and Jean-Michel Gaillard and A. Michelle Lawing and John B. Iverson and Ditte G. Christiansen and Iñigo Martínez-Solano and Gregorio Sánchez-Montes and Jorge Gutiérrez-Rodríguez and Francis L. Rose and Nicola Nelson and Susan Keall and Alain J. Crivelli and Theodoros Nazirides and Annegret Grimm-Seyfarth and Klaus Henle and Emiliano Mori and Gaëtan Guiller and Rebecca Homan and Anthony Olivier and Erin Muths and Blake R. Hossack and Xavier Bonnet and David S. Pilliod and Marieke Lettink and Tony Whitaker and Benedikt R. Schmidt and Michael G. Gardner and Marc Cheylan and Françoise Poitevin and Ana Golubović and Ljiljana Tomović and Dragan Arsovski and Richard A. Griffiths and Jan W. Arntzen and Jean-Pierre Baron and Jean-François Le Galliard and Thomas Tully and Luca Luiselli and Massimo Capula and Lorenzo Rugiero and Rebecca McCaffery and Lisa A. Eby and Venetia Briggs-Gonzalez and Frank Mazzotti and David Pearson and Brad A. Lambert and David M. Green and Nathalie Jreidini and Claudio Angelini and Graham Pyke and Jean-Marc Thirion and Pierre Joly and Jean-Paul Léna and Anton D. Tucker and Col Limpus and Pauline Priol and Aurélien Besnard and Pauline Bernard and Kristin Stanford and Richard King and Justin Garwood and Jaime Bosch and Franco L. Souza and Jaime Bertoluci and Shirley Famelli and Kurt Grossenbacher and Omar Lenzi and Kathleen Matthews and Sylvain Boitaud and Deanna H. Olson and Tim S. Jessop and Graeme R. Gillespie and Jean Clobert and Murielle Richard and Andrés Valenzuela-Sánchez and Gary M. Fellers and Patrick M. Kleeman and Brian J. Halstead and Evan H. Campbell Grant and Phillip G. Byrne and Thierry Frétey and Bernard Le Garff and Pauline Levionnois and John C. Maerz and Julian Pichenot and Kurtuluş Olgun and Nazan Üzüm and Aziz Avcı and Claude Miaud and Johan Elmberg and Gregory P. Brown and Richard Shine and Nathan F. Bendik and Lisa O’Donnell and Courtney L. Davis and Michael J. Lannoo and Rochelle M. Stiles and Robert M. Cox and Aaron M. Reedy and Daniel A. Warner and Eric Bonnaire and Kristine Grayson and Roberto Ramos-Targarona and Eyup Baskale and David Muñoz and John Measey and F. Andre Villiers and Will Selman and Victor Ronget and Anne M. Bronikowski and David A. W. Miller},

url = {https://www.science.org/doi/abs/10.1126/science.abm0151},

doi = {10.1126/science.abm0151},

year  = {2022},

date = {2022-06-01},

urldate = {2022-07-05},

journal = {Science},

volume = {376},

number = {6600},

pages = {1459--1466},

note = {Publisher: American Association for the Advancement of Science},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{dezetter_additive_2021,

title = {Additive effects of developmental acclimation and physiological syndromes on lifetime metabolic and water loss rates of a dry-skinned ectotherm},

author = {Mathias Dezetter and Andréaz Dupoué and Jean François Le Galliard and Olivier Lourdais},

url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/1365-2435.13951},

doi = {10.1111/1365-2435.13951},

issn = {1365-2435},

year  = {2022},

date = {2022-02-01},

journal = {Functional Ecology},

volume = {36},

issue = {2},

pages = {432-445},

abstract = {Developmental plasticity and thermal acclimation can contribute to adaptive responses to climate change by altering functional traits related to energy and water balance regulation. How plasticity interacts with physiological syndromes through lifetime in long-lived species is currently unknown. Here, we examined the impacts of long term thermal acclimation in a long-lived temperate ectotherm (Vipera aspis) and its potential flexibility at adulthood for two related functional traits: standard metabolic rate (SMR) and total evaporative water loss (TEWL). We used climatic chambers to simulate three contrasted daily thermal cycles (warm, medium and cold) differing in mean temperatures (28, 24, and 20°C respectively) and amplitudes (5, 10 and 13°C respectively) during immature life (0 to 4 years of age). Individuals were then maintained under common garden conditions (medium cycle) for an additional 3-years period (4 to 7 years of age). SMR and TEWL were repeatedly measured in the same individuals throughout life during and after the climate manipulation. Individuals reduced their SMR (negative compensation) when experiencing the warm cycle but flexibly adjusted their SMR to common garden conditions at adulthood. In addition, thermal conditions during the juvenile life stage led to changes in TEWL persisting until adulthood. We further found consistent intra-individual variation for SMR and TEWL and a positive intra-individual and inter-individual covariation between them throughout life. Thus, plastic responses were combined with a physiological syndrome linking SMR and TEWL. Our study demonstrates the capacity of long-lived organisms to flexibly shift their SMR to reduce daily maintenance costs in warmer and less variable thermal environments, which might be beneficial for low energy specialist organisms such as vipers. It further suggests that thermal conditions provide cues for developmental changes in TEWL. Beside plasticity, contrasted individual physiological syndromes could be selected for and contribute to the response to climate change.},

note = {_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/1365-2435.13951},

keywords = {metabolism, physiology, plasticity, temperature, water loss},

pubstate = {published},

tppubtype = {article}

}

@article{10.1242/jeb.243777,

title = {Two stressors are worse than one: combined heatwave and drought affect hydration state and glucocorticoid levels in a temperate ectotherm},

author = {Mathias Dezetter and Jean-François Le Galliard and Mathieu Leroux-Coyau and François Brischoux and Fréderic Angelier and Olivier Lourdais},

url = {https://doi.org/10.1242/jeb.243777},

doi = {10.1242/jeb.243777},

issn = {0022-0949},

year  = {2022},

date = {2022-01-01},

journal = {Journal of Experimental Biology},

abstract = {Heatwaves and droughts are becoming more intense and frequent with climate change. These extreme weather events often occur simultaneously and may alter organismal physiology, yet their combined impacts remain largely unknown. Here, we experimentally investigated physiological responses of a temperate ectotherm, the asp viper (Vipera aspis), to a simulated heatwave and drought. We applied a two-by-two factorial design by manipulating the daily temperature cycle (control vs. heatwave) and the water availability (water available vs. water-deprived) over a month followed by exposure to standard thermal conditions with ad libium access to water. Simulated heatwave and water deprivation additively increased mass loss, while water deprivation led to greater plasma osmolality (dehydration). Mass gain from drinking after the treatment period was higher in vipers from the heatwave and water-deprived group suggesting that thirst was synergistically influenced by thermal and water constraints. Heatwave conditions and water deprivation also additively increased baseline corticosterone levels but did not influence basal metabolic rates and plasma markers of oxidative stress. Our results demonstrate that a short-term exposure to combined heatwave and drought can exacerbate physiological stress through additive effects, and interactively impact behavioral responses to dehydration. Considering combined effects of temperature and water availability is thus crucial to assess organismal responses to climate change.},

note = {jeb.243777},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{badiane_environmental_2022,

title = {Environmental conditions and male quality traits simultaneously explain variation of multiple colour signals in male lizards},

author = {Arnaud Badiane and Andréaz Dupoué and Pauline Blaimont and Donald B. Miles and Anthony L. Gilbert and Mathieu Leroux-Coyau and Anna Kawamoto and David Rozen-Rechels and Sandrine Meylan and Jean Clobert and Jean-François Le Galliard},

url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/1365-2656.13773},

doi = {10.1111/1365-2656.13773},

issn = {1365-2656},

year  = {2022},

date = {2022-01-01},

urldate = {2022-08-01},

journal = {Journal of Animal Ecology},

volume = {n/a},

number = {n/a},

abstract = {Male lizards often display multiple pigment-based and structural colour signals which may reflect various quality traits (e.g. performance, parasitism), with testosterone (T) often mediating these relationships. Furthermore, environmental conditions can explain colour signal variation by affecting processes such as signal efficacy, thermoregulation, and camouflage. The relationships between colour signals, male quality traits, and environmental factors have often been analysed in isolation, but simultaneous analyses are rare. Thus, the response of multiple colour signals to variation in all these factors in an integrative analysis remains to be investigated. Here, we investigated how multiple colour signals relate to their information content, examined the role of T as a potential mediator of these relationships, and how environmental factors explain colour signal variation. We performed an integrative study to examine the covariation between three colour signals (melanin-based black, carotenoid-based yellow-orange, and structural UV), physiological performance, parasitism, T levels, and environmental factors (microclimate, forest cover) in male common lizards (Zootoca vivipara) from 13 populations. We found that the three colour signals conveyed information on different aspects of male condition, supporting a multiple message hypothesis. T influenced only parasitism, suggesting that T does not directly mediate the relationships between colour signals and their information content. Moreover, colour signals became more saturated in forested habitats, suggesting an adaptation to degraded light conditions, and became generally brighter in mesic conditions, in contradiction with the thermal melanism hypothesis. We show that distinct individual quality traits and environmental factors simultaneously explain variations of multiple colour signals with different production modes. Our study therefore highlights the complexity of colour signal evolution, involving various sets of selective pressures acting at the same time, but in different ways depending on colour production mechanism.},

note = {_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/1365-2656.13773},

keywords = {Animal communication, Coloration, Parasitism, performance, Reptile, Testosterone, Ultraviolet, Zootoca vivipara},

pubstate = {published},

tppubtype = {article}

}

@article{kawamoto_role_2021,

title = {The role of social costs as a mechanism enforcing the honesty of ultraviolet-reflecting signals in a lizard},

author = {Anna Kawamoto and Jean-François Le Galliard and Arnaud Badiane},

url = {https://doi.org/10.1093/biolinnean/blab008},

doi = {10.1093/biolinnean/blab008},

issn = {0024-4066},

year  = {2021},

date = {2021-05-13},

urldate = {2021-05-13},

journal = {Biological Journal of the Linnean Society},

volume = {133},

issue = {4},

pages = {1126–1138},

abstract = {According to animal signalling theory, social costs incurred by aggressive conspecifics are one mechanism maintaining signal honesty. Although our understanding of signal evolution has much improved for pigment-based colours, the mechanisms maintaining the honesty of structural colour signals, such as ultraviolet (UV), remain elusive. Here, we used the common lizard (Zootoca vivipara) to test whether the honesty of UV-reflecting signals displayed on male throats is under social control. To do so, we staged agonistic interactions between non-manipulated focal males and opponents of either larger or smaller body size. We manipulated the UV component of the male throat colour patch to create small cheaters with UV-enhanced throats, large cheaters with UV-reduced throats, and their respective controls. In support of a conventional signal hypothesis, focal males were aggressive towards large cheaters and became submissive when these large cheaters retaliated, and were less submissive against small cheaters. However, that focal males were not more aggressive towards small cheaters contradicts our initial predictions. We confirm that male UV reflectance and bite force were good predictors of contest outcomes in control conditions. Overall, we provide partial evidence suggesting that social costs enforce UV signal honesty in common lizards.},

keywords = {competition, sexual selection, UV coloration},

pubstate = {published},

tppubtype = {article}

}

@article{10.1371/journal.pone.0247514,

title = {Short-term changes in air humidity and water availability weakly constrain thermoregulation in a dry-skinned ectotherm},

author = {Jean-François Le Galliard and David Rozen-Rechels and Anjélica Lecomte and Clémence Demay and Andréaz Dupoué and Sandrine Meylan},

url = {https://doi.org/10.1371/journal.pone.0247514},

doi = {10.1371/journal.pone.0247514},

year  = {2021},

date = {2021-01-01},

journal = {PLOS ONE},

volume = {16},

number = {2},

pages = {1-17},

publisher = {Public Library of Science},

abstract = {Thermoregulation is critical for ectotherms as it allows them to maintain their body temperature close to an optimum for ecological performance. Thermoregulation includes a range of behaviors that aim at regulating body temperature within a range centered around the thermal preference. Thermal preference is typically measured in a thermal gradient in fully-hydrated and post-absorptive animals. Short-term effects of the hydric environment on thermal preferences in such set-ups have been rarely quantified in dry-skinned ectotherms, despite accumulating evidence that dehydration might trade-off with behavioral thermoregulation. Using experiments performed under controlled conditions in climatic chambers, we demonstrate that thermal preferences of a ground-dwelling, actively foraging lizard (Zootoca vivipara) are weakly decreased by a daily restriction in free-standing water availability (less than 0.5°C contrast). The influence of air humidity during the day on thermal preferences depends on time of the day and sex of the lizard, and is generally weaker than those of of free-standing water (less than 1°C contrast). This shows that short-term dehydration can influence, albeit weakly, thermal preferences under some circumstances in this species. Environmental humidity conditions are important methodological factors to consider in the analysis of thermal preferences.},

keywords = {lizard, thermoregulation, water balance},

pubstate = {published},

tppubtype = {article}

}

@article{https://doi.org/10.1111/ddi.13202b,

title = {Genetic and demographic trends from rear to leading edge are explained by climate and forest cover in a cold-adapted ectotherm},

author = {Andréaz Dupoué and Audrey Trochet and Murielle Richard and Mahaut Sorlin and Michaël Guillon and Jules Teulieres-Quillet and Clément Vallé and Cyrielle Rault and Maud Berroneau and Matthieu Berroneau and Olivier Lourdais and Pauline Blaimont and Romain Bertrand and Gilles Pottier and Olivier Calvez and Olivier Guillaume and Hugo Le Chevalier and Jérémie Souchet and Jean-François Le Galliard and Jean Clobert and Fabien Aubret},

url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/ddi.13202},

doi = {https://doi.org/10.1111/ddi.13202},

year  = {2021},

date = {2021-01-01},

journal = {Diversity and Distributions},

volume = {27},

number = {2},

pages = {267-281},

abstract = {Abstract Aim Determining whether altitudinal shifts in species distributions leave molecular footprints on wild populations along their range margins from rear to leading edge. Location South-west France. Methods We compared the demographic and genetic variation in 42 wild populations of the Western oviparous subclade B2 of a cold-adapted lizard (Zootoca vivipara louislantzi). These populations can be divided into four ecological units across altitudinal clines in South-west France (rear edge: <100 m, admixture zone: 100–500 m, continuous range: 500–1,300 m and leading edge: >1,300 m above sea level). Results Within the rear edge were found the highest levels of inbreeding, genetic differentiation and evidence of interrupted gene flow compared to central or colonizing areas. Within the leading edge, altitudinal range expansion occurred over the last centuries and populations showed relatively low genetic diversity. These demographic and genetic trends were better explained by inhospitable (warm and dry) climate conditions and forest cover. Main conclusions This empirical evidence illustrates that molecular footprints of climate conditions and habitat quality on wild population trends can be perceived after recent events, which should be of particular importance to accurately understand and anticipate human-induced global change on wild species and ecosystems.},

keywords = {climate change, colonization, ectotherms, gene flow, inbreeding, structuration},

pubstate = {published},

tppubtype = {article}

}

@article{https://doi.org/10.1111/gcb.15471,

title = {Ecotrons: Powerful and versatile ecosystem analysers for ecology, agronomy and environmental science},

author = {Jacques Roy and François Rineau and Hans J De Boeck and Ivan Nijs and Thomas Pütz and Samuel Abiven and John A Arnone III and Craig V M Barton and Natalie Beenaerts and Nicolas Brüggemann and Matteo Dainese and Timo Domisch and Nico Eisenhauer and Sarah Garré and Alban Gebler and Andrea Ghirardo and Richard L Jasoni and George Kowalchuk and Damien Landais and Stuart H Larsen and Vincent Leemans and Jean-François Le Galliard and Bernard Longdoz and Florent Massol and Teis N Mikkelsen and Georg Niedrist and Clément Piel and Olivier Ravel and Joana Sauze and Anja Schmidt and Jörg-Peter Schnitzler and Leonardo H Teixeira and Mark G Tjoelker and Wolfgang W Weisser and Barbro Winkler and Alexandru Milcu},

url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/gcb.15471},

doi = {https://doi.org/10.1111/gcb.15471},

year  = {2021},

date = {2021-01-01},

urldate = {2021-01-01},

journal = {Global Change Biology},

volume = {27},

number = {7},

pages = {1387-1407},

abstract = {Abstract Ecosystems integrity and services are threatened by anthropogenic global changes. Mitigating and adapting to these changes require knowledge of ecosystem functioning in the expected novel environments, informed in large part through experimentation and modelling. This paper describes 13 advanced controlled environment facilities for experimental ecosystem studies, herein termed ecotrons, open to the international community. Ecotrons enable simulation of a wide range of natural environmental conditions in replicated and independent experimental units while measuring various ecosystem processes. This capacity to realistically control ecosystem environments is used to emulate a variety of climatic scenarios and soil conditions, in natural sunlight or through broad-spectrum lighting. The use of large ecosystem samples, intact or reconstructed, minimizes border effects and increases biological and physical complexity. Measurements of concentrations of greenhouse trace gases as well as their net exchange between the ecosystem and the atmosphere are performed in most ecotrons, often quasi continuously. The flow of matter is often tracked with the use of stable isotope tracers of carbon and other elements. Equipment is available for measurements of soil water status as well as root and canopy growth. The experiments ran so far emphasize the diversity of the hosted research. Half of them concern global changes, often with a manipulation of more than one driver. About a quarter deal with the impact of biodiversity loss on ecosystem functioning and one quarter with ecosystem or plant physiology. We discuss how the methodology for environmental simulation and process measurements, especially in soil, can be improved and stress the need to establish stronger links with modelling in future projects. These developments will enable further improvements in mechanistic understanding and predictive capacity of ecotron research which will play, in complementarity with field experimentation and monitoring, a crucial role in exploring the ecosystem consequences of environmental changes.},

keywords = {biodiversity, controlled environment facilities, ecosystem functioning, ecosystem process measurements, environmental simulations, experimentation, global change, research infrastructures},

pubstate = {published},

tppubtype = {article}

}

@article{rutschmann_intense_2021,

title = {Intense nocturnal warming alters growth strategies, coloration, and parasite load in a diurnal lizard},

author = {Alexis Rutschmann and Andréaz Dupoué and Donald B. Miles and Megía R. Palma and C Lauden and Murielle Richard and Arnaud Badiane and David Rozen‐Rechels and Mathieu Brevet and Pauline Blaimont and Sandrine Meylan and Jean Clobert and Jean-François Le Galliard},

url = {https://besjournals.onlinelibrary.wiley.com/doi/abs/10.1111/1365-2656.13502},

doi = {https://doi.org/10.1111/1365-2656.13502},

issn = {1365-2656},

year  = {2021},

date = {2021-01-01},

urldate = {2021-01-01},

journal = {Journal of Animal Ecology},

volume = {90},

number = {8},

pages = {1864-1877},

abstract = {1. In the past decades, nocturnal temperatures have been playing a disproportionate role in the global warming of the planet. Yet, they remain a neglected factor in studies assessing the impact of global warming on natural populations. 2. Here, we question whether an intense augmentation of nocturnal temperatures is beneficial or deleterious to ectotherms. Physiological performance is influenced by thermal conditions in ectotherms and an increase in temperature by only 2°C is sufficient to induce a disproportionate increase in metabolic expenditure. Warmer nights may expand ectotherms’ species thermal niche and open new opportunities for prolonged activities and improve foraging efficiency. However, increased activity may also have deleterious effects on energy balance if exposure to warmer nights reduces resting periods and elevates resting metabolic rate. 3. We assessed whether warmer nights affected an individual’s growth, dorsal skin colouration, thermoregulation behaviour, oxidative stress status and parasite load by exposing yearling common lizards (Zootoca vivipara) from four populations to either ambient or high nocturnal temperatures for approximately five weeks. 4. Warmer nocturnal temperatures increased the prevalence of ectoparasitic infestation and altered allocation of resources toward structural growth rather than storage. We found no change in markers for oxidative stress. The thermal treatment did not influence thermal preferences, but influenced dorsal skin brightness and luminance, in line with a predicted acclimation response in colder environments to enhance heat gain from solar radiation. 5. Altogether, our results highlight the importance of considering nocturnal warming as an independent factor affecting ectotherms life-history in the context of global climate change.},

keywords = {coloration change, ectotherms, energetic balance, nocturnal temperatures, oxidative stress},

pubstate = {published},

tppubtype = {article}

}

@article{le_galliard_worldwide_2021,

title = {A worldwide and annotated database of evaporative water loss rates in squamate reptiles},

author = {J. -F. Le Galliard and Chloé Chabaud and Denis Otávio Vieira de Andrade and Franc cois Brischoux and Miguel A. Carretero and Andréaz Dupoué and Rodrigo S. B. Gavira and Olivier Lourdais and Marco Sannolo and Tom J. M. Van Dooren},

url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/geb.13355},

doi = {10.1111/geb.13355},

issn = {1466-8238},

year  = {2021},

date = {2021-01-01},

urldate = {2021-01-01},

journal = {Global Ecology and Biogeography},

volume = {30},

number = {10},

pages = {1938--1950},

abstract = {Motivation The understanding of physiological adaptations, of evolutionary radiations and of ecological responses to global change urges for global, comprehensive databases of the functional traits of extant organisms. The ability to maintain an adequate water balance is a critical functional property influencing the resilience of animal species to climate variation. In terrestrial or semi-terrestrial organisms, total water loss includes a significant contribution from evaporative water loss (EWL). The analysis of geographic and phylogenetic variation in EWL rates must however account for differences in methods and potential confounding factors, which influence standard measures of whole-organism water loss. We compiled the global and standardized SquamEWL database of total, respiratory and cutaneous EWL for 325 species and subspecies of squamate reptiles (793 samples and 2,536 estimates) from across the globe. An extensive set of companion data and annotations associated with the EWL measurements of potential value for future investigation, including metabolic rate data, is provided. We present preliminary descriptive statistics for the compiled data, discuss gaps and biases, and identify promising avenues to update, expand and explore this database. Main types of variables contained Standard water loss rates, geographic data, metabolic rates. Spatial location Global. Time period Data were obtained from extant species and were collected between 1945 and 2020. Major taxa Reptilia, Squamata including lizards, snakes and amphisbaenians. Level of measurements Individual samples of animals from the same species, locality, age class and sex category. Software format csv.},

note = {_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/geb.13355},

keywords = {ectotherms, evaporative water loss, functional traits, homeostasis, hydroregulation, lizards, macrophysiology, snakes, vertebrate macrophysiology, water loss},

pubstate = {published},

tppubtype = {article}

}

@article{dezetter_water_2021,

title = {Water deprivation compromises maternal physiology and reproductive success in a cold and wet adapted snake Vipera berus},

author = {Mathias Dezetter and Jean François Le Galliard and Gaëtan Guiller and Michaël Guillon and Mathieu Leroux-Coyau and Sandrine Meylan and François Brischoux and Fréderic Angelier and Olivier Lourdais},

url = {https://doi.org/10.1093/conphys/coab071},

doi = {10.1093/conphys/coab071},

issn = {2051-1434},

year  = {2021},

date = {2021-01-01},

urldate = {2021-09-09},

journal = {Conservation Physiology},

volume = {9},

number = {1},

abstract = {Droughts are becoming more intense and frequent with climate change. These extreme weather events can lead to mass mortality and reproduction failure, and therefore cause population declines. Understanding how the reproductive physiology of organisms is affected by water shortages will help clarify whether females can adjust their reproductive strategy to dry conditions or may fail to reproduce and survive. In this study, we investigated the consequences of a short period of water deprivation (2 weeks) during early pregnancy on the physiology and behaviour of a cold- and wet-adapted ectotherm (Vipera berus). We also examined water allocation to developing embryos and embryonic survival. Water-deprived females exhibited significant dehydration, physiological stress and loss of muscle mass. These effects of water deprivation on water balance and muscle loss were correlated with the number of developing embryos. While water-deprived females maintained water transfer to embryos at the expense of their own maintenance, water deprivation also led to embryonic mortality. Overall, water deprivation amplifies the reproductive costs of water allocation to support embryonic development. The deleterious impacts of water deprivation on female current reproductive performance and on potential survival and future reproduction could lead to severe population declines in this species.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{rozenrechels_interaction_2021,

title = {Interaction of hydric and thermal conditions drive geographic variation in thermoregulation in a widespread lizard},

author = {David Rozen‐Rechels and Alexis Rutschmann and Andréaz Dupoué and Pauline Blaimont and Victor Chauveau and Donald B. Miles and Michael Guillon and Murielle Richard and Arnaud Badiane and Sandrine Meylan and Jean Clobert and Jean-François Le Galliard},

url = {https://esajournals.onlinelibrary.wiley.com/doi/abs/10.1002/ecm.1440},

doi = {10.1002/ecm.1440},

issn = {1557-7015},

year  = {2021},

date = {2021-01-01},

urldate = {2020-11-05},

journal = {Ecological Monographs},

volume = {91},

number = {2},

pages = {e01440},

abstract = {Behavioral thermoregulation is an efficient mechanism to buffer the physiological effects of climate change. Thermal ecology studies have traditionally tested how thermal constraints shape thermoregulatory behaviors without accounting for the potential major effects of landscape structure and water availability. Thus, we lack a general understanding of the multifactorial determinants of thermoregulatory behaviors in natural populations. In this study, we quantified the relative contribution of elevation, thermal gradient, moisture gradient and landscape structure in explaining geographic variation in thermoregulation strategies of a terrestrial ectotherm species. We measured field active body temperature, thermal preferences and operative environmental temperatures to calculate thermoregulation indices, including thermal quality of the habitat and thermoregulation efficiency for a very large sample of common lizards (Zootoca vivipara) from 21 populations over 3 years across the Massif Central mountain range in France. We used an information-theoretic approach to compare eight a priori thermo-hydroregulation hypotheses predicting how behavioral thermoregulation should respond to environmental conditions. Environmental characteristics exerted little influence on thermal preference with the exception that females from habitats with permanent access to water had lower thermal preferences. Field body temperatures and accuracy of thermoregulation were best predicted by the interaction between air temperature and a moisture index. In mesic environments, field body temperature and thermoregulation inaccuracy increased with air temperature, but they decreased in drier habitats. Thermoregulation efficiency (difference between thermoregulation inaccuracy and the thermal quality of the habitat) was maximized in cooler and more humid environments and was mostly influenced by the thermal quality of the habitat. Our study highlights complex patterns of variation in thermoregulation strategies, which are mostly explained by the interaction between temperature and water availability, independent of the elevation gradient or thermal heterogeneity. Although changes in landscape structure were expected to be the main driver of extinction rate of temperate zone ectotherms with ongoing global change, we conclude that changes in water availability coupled with rising temperatures might have a drastic impact on the population dynamics of some ectotherm species.},

keywords = {body temperature, elevational gradient, landscape heterogeneity, moisture gradient, non-avian reptiles},

pubstate = {published},

tppubtype = {article}

}

@article{dupoue_water_2020,

title = {Water availability and temperature induce changes in oxidative status during pregnancy in a viviparous lizard},

author = {Andréaz Dupoué and Pauline Blaimont and David Rozen‐Rechels and Murielle Richard and Sandrine Meylan and Jean Clobert and Donald B Miles and Rémi Martin and Beatriz Decencière and Simon Agostini and Jean‐François Le Galliard},

editor = {David Costantini},

url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/1365-2435.13481},

doi = {10.1111/1365-2435.13481},

issn = {0269-8463, 1365-2435},

year  = {2020},

date = {2020-02-01},

urldate = {2020-02-11},

journal = {Functional Ecology},

volume = {34},

number = {2},

pages = {475-485},

keywords = {antioxidant capacity, ectotherms, oxidative stress, performance, temperature},

pubstate = {published},

tppubtype = {article}

}

@article{dupoue_chronic_2020,

title = {Chronic water restriction triggers sex-specific oxidative stress and telomere shortening in lizards},

author = {Andréaz Dupoué and Frédéric Angelier and Cécile Ribout and Sandrine Meylan and David Rozen-Rechels and Beatriz Decencière and Simon Agostini and Jean-François Le Galliard},

url = {https://royalsocietypublishing.org/doi/10.1098/rsbl.2019.0889},

doi = {10.1098/rsbl.2019.0889},

issn = {1744-9561, 1744-957X},

year  = {2020},

date = {2020-01-01},

urldate = {2020-02-26},

journal = {Biology Letters},

volume = {16},

number = {2},

pages = {20190889},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{dusza_biotic_2020,

title = {Biotic soil-plant interaction processes explain most of hysteric soil CO2 efflux response to temperature in cross-factorial mesocosm experiment},

author = {Yann Dusza and Enrique P Sanchez-Cañete and Jean-François Le Galliard and Régis Ferrière and Simon Chollet and Florent Massol and Amandine Hansart and Sabrina Juarez and Katerina Dontsova and Joost van Haren and Peter Troch and Mitchell A Pavao-Zuckerman and Erik Hamerlynck and Greg A Barron-Gafford},

url = {https://www.nature.com/articles/s41598-019-55390-6},

doi = {10.1038/s41598-019-55390-6},

issn = {2045-2322},

year  = {2020},

date = {2020-01-01},

urldate = {2020-01-22},

journal = {Scientific Reports},

volume = {10},

number = {1},

pages = {1--11},

abstract = {Ecosystem carbon flux partitioning is strongly influenced by poorly constrained soil CO2 efflux (Fsoil). Simple model applications (Arrhenius and Q10) do not account for observed diel hysteresis between Fsoil and soil temperature. How this hysteresis emerges and how it will respond to variation in vegetation or soil moisture remains unknown. We used an ecosystem-level experimental system to independently control potential abiotic and biotic drivers of the Fsoil-T hysteresis. We hypothesized a principally biological cause for the hysteresis. Alternatively, Fsoil hysteresis is primarily driven by thermal convection through the soil profile. We conducted experiments under normal, fluctuating diurnal soil temperatures and under conditions where we held soil temperature near constant. We found (i) significant and nearly equal amplitudes of hysteresis regardless of soil temperature regime, and (ii) the amplitude of hysteresis was most closely tied to baseline rates of Fsoil, which were mostly driven by photosynthetic rates. Together, these findings suggest a more biologically-driven mechanism associated with photosynthate transport in yielding the observed patterns of soil CO2 efflux being out of sync with soil temperature. These findings should be considered on future partitioning models of ecosystem respiration.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{josserand_chronic_2020,

title = {Chronic elevation of glucorticoids late in life generates long lasting changes in physiological state without a life history switch},

author = {Rémy Josserand and Claudy Haussy and Simon Agostini and Beatriz Decencière and Jean-François Le Galliard and Sandrine Meylan},

url = {http://www.sciencedirect.com/science/article/pii/S0016648019300929},

doi = {10.1016/j.ygcen.2019.113288},

issn = {0016-6480},

year  = {2020},

date = {2020-01-01},

urldate = {2019-09-26},

journal = {General and Comparative Endocrinology},

volume = {285},

pages = {113288},

abstract = {Chronic stressors have profound impacts on phenotypes and life history strategies on the short term, but delayed effects of stress experienced late in life remain poorly investigated in wild populations. Here, we used a combined laboratory and field experiment to test if chronic stress late in life has immediate and delayed effects on physiological and demographic traits in the common lizard, Zootoca vivipara. We increased plasma corticosterone levels in adults and yearlings during three weeks of the post-reproductive season. We quantified immediate responses in the laboratory, delayed intra-generational effects in field enclosures one month and one year later during the next reproductive season, and delayed inter-generational effects in the first generation of offspring. Our phenotypic assays included metabolism, immune capacities, lipid metabolism and oxidative stress. Relative to placebos, lizards treated with corticosterone had higher body condition and lower oxidative damages but an increased skin swelling response directly after the manipulation. Delayed responses in field enclosures were of three types. First, we found catch-up growth for body mass such the placebos had similar body conditions one month after the laboratory manipulation. Second, we found persistent differences in oxidative damages during one month but not one year later. Third, during the next reproductive season, corticosterone-treated females had higher levels of plasma triglycerides, whereas corticosterone-treated individuals had a higher skin swelling response. We found no delayed inter-generational effects on demographic traits of offspring. Our study demonstrates the potential for long-lasting physiological consequences of chronic corticosterone enhancement despite no obvious changes in life history.},

keywords = {immunity, maternal effect, metabolism, oxidative stress, stress, triglycerides},

pubstate = {published},

tppubtype = {article}

}

@article{rozen-rechels_water_2020,

title = {Water restriction induces behavioral fight but impairs thermoregulation in a dry-skinned ectotherm},

author = {David Rozen-Rechels and Arnaud Badiane and Simon Agostini and Sandrine Meylan and Jean-François Le Galliard},

url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/oik.06910},

doi = {10.1111/oik.06910},

issn = {1600-0706},

year  = {2020},

date = {2020-01-01},

urldate = {2020-02-11},

journal = {Oikos},

volume = {129},

number = {4},

pages = {572--584},

abstract = {Behavioral fight responses to desiccation risk are important to predict the vulnerability of terrestrial animals to climate change and yet, they have received little attention so far. In terrestrial ectotherms, behavioral regulation of the water balance (i.e. hydroregulation) is likely to be plastic and may tradeoff with thermoregulation behavior because water loss rates are generally higher in warmer environments and body temperatures. When low water availability and heat stress cause physiological dehydration, we expect to highlight a shift to behavioral water-conservation strategies including changes in activity patterns, micro-habitat selection and thermoregulation strategies. Here, we compared the behavior of adult common lizards Zootoca vivipara in indoor arenas that either had a permanent access to water or underwent a one-week long experimental water restriction. Water-restricted lizards reduced their behavioral activity, selected more often cooler and wetter refuges during daytime, and performed less accurate thermoregulation than control lizards. The activity of water-restricted gravid females shifted towards the cooler and wetter early hours of the day. In addition, they had lower body temperatures and preferred lower body temperatures at the end of the experiment (i.e. thermal depression). Water-restricted lizards suffered from a mild physiological dehydration and had a lower mass change. Heat stress was simulated every second day, which led to a range of heat avoidance and water conservation strategies independent from water restriction. Altogether, these results confirm that chronic water restriction and dehydration induce responses towards water conservation that conflict with thermoregulation accuracy.},

keywords = {activity, body temperature, dehydration, space use, squamate reptiles, water availability},

pubstate = {published},

tppubtype = {article}

}

@article{rozen-rechels_acclimation_2020,

title = {Acclimation to water restriction implies different paces for behavioral and physiological responses in a lizard species},

author = {David Rozen-Rechels and Andréaz Dupoué and Sandrine Meylan and Kenza Qitout and Beatriz Decencière and Simon Agostini and Jean-François Le Galliard},

url = {https://www.journals.uchicago.edu/doi/abs/10.1086/707409},

doi = {10.1086/707409},

issn = {1522-2152},

year  = {2020},

date = {2020-01-01},

urldate = {2019-12-11},

journal = {Physiological and Biochemical Zoology},

volume = {93},

number = {2},

pages = {160--174},

keywords = {activity, body temperature, exploration, metabolism, squamate reptiles, water availability, water loss},

pubstate = {published},

tppubtype = {article}

}

@article{dupoue_mother-offspring_2020,

title = {Mother-offspring conflict for water and its mitigation in the oviparous form of the reproductively bimodal lizard, Zootoca vivipara},

author = {Andréaz Dupoué and Mahaut Sorlin and Murielle Richard and Jean-François Le Galliard and Olivier Lourdais and Jean Clobert and Fabien Aubret},

url = {https://academic.oup.com/biolinnean/article-abstract/129/4/888/5809298?redirectedFrom=fulltext},

doi = {https://doi.org/10.1093/biolinnean/blaa012},

year  = {2020},

date = {2020-01-01},

journal = {Biological Journal of the Linnean Society},

volume = {129},

number = {4},

pages = {888--900},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{badiane_male_2020,

title = {Male ultraviolet reflectance and female mating history influence female mate choice and male mating success in a polyandrous lizard},

author = {Arnaud Badiane and Mélissa Martin and Sandrine Meylan and Murielle Richard and Beatriz Decencière Ferrandière and Jean-François Le Galliard},

url = {https://academic.oup.com/biolinnean/article-abstract/130/3/586/5847665?redirectedFrom=fulltext},

doi = {https://doi.org/10.1093/biolinnean/blaa061},

year  = {2020},

date = {2020-01-01},

journal = {Biological Journal of the Linnean Society},

volume = {130},

number = {3},

pages = {586--598},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{rozenrechels_short-term_2020,

title = {Short-term change in water availability influences thermoregulation behaviours in a dry-skinned ectotherm},

author = {David Rozen‐Rechels and Pauline Farigoule and Simon Agostini and Arnaud Badiane and Sandrine Meylan and Jean-François Le Galliard},

url = {https://besjournals.onlinelibrary.wiley.com/doi/abs/10.1111/1365-2656.13279},

doi = {10.1111/1365-2656.13279},

issn = {1365-2656},

year  = {2020},

date = {2020-01-01},

urldate = {2020-08-03},

journal = {Journal of Animal Ecology},

volume = {89},

number = {9},

pages = {2099-2110},

abstract = {Mechanistic models of terrestrial ectotherms predict that climate warming will induce activity restriction due to heat stress and loss of shade, leading to the extinction of numerous populations. Such models rely on the assumption that activity patterns are dictated by simple temperature thresholds independent of changes in water availability. However, changes in water availability may further influence thermoregulation behaviour of ectotherms through dehydration risk perception, changes in water balance or changes in microclimatic conditions. Here, we experimentally assess the interactive effects of thermal conditions and water availability on activity patterns, shade selection and thermoregulation efficiency in a model ectothermic species. Thermoregulation behaviour of adult common lizards Zootoca vivipara was monitored in outdoor mesocosms as we manipulated water availability, providing water as mist in the morning and free-standing water during the daytime. We recorded operative temperatures and micro-meteorological conditions to infer thermal constraints and dehydration risk. Activity and shade selection were better predicted by continuous changes in thermal conditions and dehydration risk, respectively, than by threshold functions. In addition, water supplementation increased activity in males and reduced shade selection in both sexes, most probably as a behavioural response to the perception of a stronger dehydration risk. Water supplementation also influenced the thermal quality of the environment, which in turn altered daily activity patterns and thermoregulation statistics. This demonstrates that dual effects of heat and water stress on activity patterns may lead to stronger activity restriction as a result of climate change than currently predicted.},

keywords = {activity, body temperature, dehydration, squamate reptiles, thermoregulation, water availability},

pubstate = {published},

tppubtype = {article}

}

@article{Bruschjeb228064,

title = {Additive effects of temperature and water availability on pregnancy in a viviparous lizard},

author = {George A Brusch and Rodrigo S B Gavira and Robin Viton and Andréaz Dupoué and Mathieu Leroux-Coyau and Sandrine Meylan and Jean-Fran{c c}ois Le Galliard and Olivier Lourdais},

url = {https://jeb.biologists.org/content/223/19/jeb228064},

doi = {10.1242/jeb.228064},

issn = {0022-0949},

year  = {2020},

date = {2020-01-01},

journal = {Journal of Experimental Biology},

volume = {223},

number = {19},

publisher = {The Company of Biologists Ltd},

abstract = {One of the greatest current threats to biodiversity is climate change. However, understanding of organismal responses to fluctuations in temperature and water availability is currently lacking, especially during fundamental life-history stages such as reproduction. To further explore how temperature and water availability impact maternal physiology and reproductive output, we used the viviparous form of the European common lizard (Zootoca vivipara) in a two-by-two factorial design manipulating both hydric and thermal conditions, for the first time. We collected blood samples and morphological measurements during early pregnancy and post-parturition to investigate how water availability, temperature and a combination of the two influence maternal phenology, morphology, physiology and reproductive output. We observed that dehydration during gestation negatively affects maternal physiological condition (lower mass gain, higher tail reserve mobilization) but has little effect on reproductive output. These effects are mainly additive to temperature regimes, with a proportional increase in maternal costs in warmer environments. Our study demonstrates the importance of considering combined effects of water and temperature when investigating organismal responses to climate changes, especially during periods crucial for species survival such as reproduction.},

keywords = {antioxidant capacity, dehydration, oxidative stress, reproduction, temperature},

pubstate = {published},

tppubtype = {article}

}

@article{https://doi.org/10.1111/1365-2656.13317,

title = {Micro-geographic shift between negligible and actuarial senescence in a wild snake},

author = {Thomas Tully and Jean-François Le Galliard and Jean-Pierre Baron},

url = {https://besjournals.onlinelibrary.wiley.com/doi/abs/10.1111/1365-2656.13317},

doi = {https://doi.org/10.1111/1365-2656.13317},

year  = {2020},

date = {2020-01-01},

journal = {Journal of Animal Ecology},

volume = {89},

number = {11},

pages = {2704-2716},

abstract = {Abstract While it has long been known that species have contrasted life expectancy (pace of mortality) and generation time (pace of reproduction), recent studies have also uncovered that the shape of adult age trajectories of mortality and reproduction can vary remarkably among species along a continuum of senescence ranging from strong deterioration (senescence), insignificant deterioration (negligible senescence) to improvement with advancing age (negative senescence). As for many long-lived ectotherms with asymptotic growth and increasing reproductive output with age, snakes are good candidates for negligible senescence to occur. Yet, intraspecific variation in the pace and shape of actuarial and reproductive senescence across wild populations of these species remains to be explored. Here, we used 37 years of mark–recapture data in two nearby habitats inside a meadow viper Vipera ursinii population to quantify life expectancies, generation times and the shape of actuarial and reproductive senescence. Female vipers maintained stable reproductive performances at old ages, even when accounting for the predicted increase of fertility with body size, providing evidence for negligible reproductive senescence in both habitats. Males had a higher adult mortality and a shorter life expectancy on average than females and actuarial senescence shifted from negligible senescence in the optimal habitat to strong senescence in the sub-optimal habitat. Overall, these results demonstrate that micro-geographic environmental variation can generate qualitative shifts in actuarial senescence patterns. This highlights that taking into account the within-species plasticity of age-dependent trajectories could prove useful in better understanding what determines the evolution of life-history age trajectories.},

keywords = {ageing, life-history trade-off, pace and shape, phenotypic plasticity, reproductive effort, survival},

pubstate = {published},

tppubtype = {article}

}

@article{names_relative_2019,

title = {The relative importance of body size and UV coloration in influencing male-male competition in a lacertid lizard},

author = {Gabrielle Names and Mélissa Martin and Arnaud Badiane and Jean-François Le Galliard},

url = {https://link.springer.com/article/10.1007/s00265-019-2710-z},

doi = {https://doi.org/10.1007/s00265-019-2710-z},

issn = {1432-0762},

year  = {2019},

date = {2019-01-01},

urldate = {2020-01-22},

journal = {Behavioral Ecology and Sociobiology},

volume = {73},

number = {7},

pages = {98},

abstract = {Communication via color signals is common in natural systems. Ultraviolet (UV)-blue patches located on the outer-ventral scales of some lacertid lizards are thought to be involved in male-male competition. However, the mechanisms that maintain their honesty remain unknown. Here, we use the common wall lizard Podarcis muralis to test whether the lateral UV-blue spots are conventional signals, the honesty of which is guaranteed by receiver-dependent costs, and discuss their potential role as an amplifier of body size. We first described the morphology and reflectance properties of lateral UV-blue spots in common wall lizards and investigated how they influence male-male competition. Spot size and number, UV chroma, and conspicuousness (calculated using vision models) were significantly greater in adult males relative to adult females and adult males relative to juveniles. Total spot area (and not spot number) of adult males was positively correlated with body size. We conducted staged competition encounters between focal males and smaller or larger rivals with control or manipulated spots. Spots were enlarged in small rivals and reduced in large rivals to disrupt the phenotypic correlation between spot area and body size. Aggressiveness and dominance were positively influenced by body size in control encounters. Spot manipulations resulted in greater submission and less aggressiveness in focal males. These results contradict the predictions associated with conventional signals and amplifiers, but suggest that spots contributed to opponent evaluation during short-distance encounters between competing males.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{dupoue_like_2019,

title = {Some like it dry: Water restriction overrides heterogametic sex determination in two reptiles},

author = {Andréaz Dupoué and Olivier Lourdais and Sandrine Meylan and François Brischoux and Frédéric Angelier and David Rozen-Rechels and Yoan Marcangeli and Béatriz Decencière and Simon Agostini and Jean-François Le Galliard},

url = {https://onlinelibrary.wiley.com/doi/full/10.1002/ece3.5229},

doi = {https://doi.org/10.1002/ece3.5229},

year  = {2019},

date = {2019-01-01},

journal = {Ecology and Evolution},

volume = {9},

number = {11},

pages = {6524--6533},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{rozen-rechels_when_2019,

title = {When water interacts with temperature: Ecological and evolutionary implications of thermo-hydroregulation in terrestrial ectotherms},

author = {David Rozen-Rechels and Andréaz Dupoué and Olivier Lourdais and Simon Chamaillé-Jammes and Sandrine Meylan and Jean Clobert and Jean-François Le Galliard},

url = {https://onlinelibrary.wiley.com/doi/full/10.1002/ece3.5440},

doi = {https://doi.org/10.1002/ece3.5440},

year  = {2019},

date = {2019-01-01},

journal = {Ecology and evolution},

volume = {9},

number = {17},

pages = {10029--10043},

keywords = {behaviour, body temperature, performance, physiological adjustments, water balance},

pubstate = {published},

tppubtype = {article}

}

@article{clobert_how_2018,

title = {How to Integrate Experimental Research Approaches in Ecological and Environmental Studies: AnaEE France as an Example},

author = {Jean Clobert and André Chanzy and Jean-François Le Galliard and Abad Chabbi and Lucile Greiveldinger and Thierry Caquet and Michel Loreau and Christian Mougin and Christian Pichot and Jacques Roy and Laurent Saint-André},

url = {https://www.frontiersin.org/articles/10.3389/fevo.2018.00043/full?&utm_source=Email_to_authors_&utm_medium=Email&utm_content=T1_11.5e1_author&utm_campaign=Email_publication&field=&journalName=Frontiers_in_Ecology_and_Evolution&id=255505},

doi = {10.3389/fevo.2018.00043},

issn = {2296-701X},

year  = {2018},

date = {2018-01-01},

urldate = {2018-04-21},

journal = {Frontiers in Ecology and Evolution},

volume = {6},

abstract = {Human activities have altered continental ecosystems worldwide and generated a major environmental crisis, prompting urgent societal questions on how to best produce goods while at the same time securing sustainable ecological services and raising needs to better understand and predict biodiversity and ecosystems dynamics under global changes. To tackle these questions, experimentation on ecosystems is necessary to improve our knowledge of processes and to propose scientifically sound management strategies. Experimental platforms able to manipulate key factors of global change and including state of the art observation methodologies are available worldwide but how to best integrate them has been rarely addressed. Here, we present and discuss the case of the national research infrastructure AnaEE France dedicated to the study of continental ecosystems and designed to congregate complementary experimental approaches in order to facilitate their access and use through a range of distributed and shared services. The conceptual design of AnaEE France includes five modules. Three modules gather experimental facilities along a gradient of experimental control ranging from highly controlled Ecotron facilities, semi-natural field mesocosms to in natura experimental sites covering major continental ecosystems (forests, croplands, grasslands and lakes). In addition, AnaEE France also includes shared instruments that can be implemented in experiments and analytical platforms specifically dedicated to environmental biology. To promote reuse of data, generalize results and improve predictive models, AnaEE France further gathers modelling and information systems. The implementation of AnaEE France allowed for mutual synergies, improved the technical skills, stimulated new experiments and helped our scientific community to enter into the big data sharing era.},

keywords = {climate change, information system, modelling, open-access platforms, research infrastructure},

pubstate = {published},

tppubtype = {article}

}

@article{rozen-rechels_water_2018,

title = {Water restriction in viviparous lizards causes transgenerational effects on behavioral anxiety and immediate effects on exploration behavior},

author = {David Rozen-Rechels and Andréaz Dupoué and Sandrine Meylan and Beatriz Decencière and Sophie Guingand and Jean-François Le Galliard},

url = {https://doi.org/10.1007/s00265-018-2443-4},

doi = {10.1007/s00265-018-2443-4},

issn = {1432-0762},

year  = {2018},

date = {2018-01-01},

urldate = {2019-09-13},

journal = {Behavioral Ecology and Sociobiology},

volume = {72},

number = {2},

pages = {23},

abstract = {Behavioral plasticity induced by maternal effects is crucial in adjusting offspring phenotype to match the environment. In particular, changes in water availability during development may initiate a range of behavioral responses, such as natal dispersal, but the contribution of maternal effects from water stress in explaining behavioral variation has been overlooked so far. In this study, we examined behavioral plasticity of juvenile common lizards (Zootoca vivipara) in response to changes in pre-natal and post-natal hydric conditions. We exposed pregnant mothers either to a moderate water restriction or to ad libitum access to water. We used neutral arena tests to measure repeatedly activity and space use behaviors of their offspring, either in dry or wet soil conditions, at two distinct life stages (juveniles and yearlings) in order to quantify behavioral flexibility, age differences, and personalities (consistent inter-individual differences). Juveniles and yearlings had greater exploration scores in dry than wet conditions regardless of the pre-natal conditions. Pre-natal water restriction and dry post-natal conditions enhanced thigmotaxis (i.e., a behavior related to anxiety) in juveniles. Maternal environment did not influence behavioral flexibility nor the behavior of yearlings. Behaviors were barely repeatable in juveniles and inconsistent through early life. Assuming that dry conditions may initiate flight responses, these results suggest complex relationships between the developmental timing of water stress, exploration and anxiety behaviors, and dispersal syndromes.Significance statementWater restriction should initiate a range of behaviors related to a flight response. However, there is little evidence that changes in water availability across development influence offspring behavior in wild animals. Experiments with lizards demonstrate that maternal water restriction increased the anxiety of their juveniles, whereas their exploration was higher in dry environments. Thus, water availability may be a critical determinant of space use behavior and dispersal syndromes.},

keywords = {maternal effect, squamate reptiles, stress, thigmotaxis, water availability},

pubstate = {published},

tppubtype = {article}

}

@article{milcu_genotypic_2018,

title = {Genotypic variability enhances the reproducibility of an ecological study},

author = {Alexandru Milcu and Ruben Puga-Freitas and Aaron M Ellison and Manuel Blouin and Stefan Scheu and Grégoire T Freschet and Laura Rose and Sebastien Barot and Simone Cesarz and Nico Eisenhauer and Thomas Girin and Davide Assandri and Michael Bonkowski and Nina Buchmann and Olaf Butenschoen and Sébastien Devidal and Gerd Gleixner and Arthur Gessler and Agnès Gigon and Anna Greiner and Carlo Grignani and Amandine Hansart and Zachary Kayler and Markus Lange and Jean-Christophe Lata and Jean-François Le Galliard and Martin Lukac and Neringa Mannerheim and Marina E. H. Müller and Anne Pando and Paula Rotter and Michael Scherer-Lorenzen and Rahme Seyhun and Katherine Urban-Mead and Alexandra Weigelt and Laura Zavattaro and Jacques Roy},

url = {https://www.nature.com/articles/s41559-017-0434-x},

doi = {https://doi.org/10.1038/s41559-017-0434-x},

year  = {2018},

date = {2018-01-01},

journal = {Nature ecology & evolution},

volume = {2},

number = {2},

pages = {279},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{dupoue_water_2018,

title = {Water restriction causes an intergenerational trade-off and delayed mother–offspring conflict in a viviparous lizard},

author = {Andréaz Dupoué and Jean-François Le Galliard and Rémy Josserand and Dale F DeNardo and Béatriz Decencière and Simon Agostini and Claudy Haussy and Sandrine Meylan},

url = {https://besjournals.onlinelibrary.wiley.com/doi/full/10.1111/1365-2435.13009},

doi = {https://doi.org/10.1111/1365-2435.13009},

year  = {2018},

date = {2018-01-01},

journal = {Functional ecology},

volume = {32},

number = {3},

pages = {676--686},

keywords = {corticosterone, dehydration, reproduction, survival, trade-off},

pubstate = {published},

tppubtype = {article}

}

@article{dupoue_reduction_2018,

title = {Reduction in baseline corticosterone secretion correlates with climate warming and drying across wild lizard populations},

author = {Andréaz Dupoué and Alexis Rutschmann and Jean-François Le Galliard and Jean Clobert and Pauline Blaimont and Barry Sinervo and Donald B Miles and Claudy Haussy and Sandrine Meylan},

url = {https://besjournals.onlinelibrary.wiley.com/doi/abs/10.1111/1365-2656.12843},

doi = {https://doi.org/10.1111/1365-2656.12843},

year  = {2018},

date = {2018-01-01},

journal = {Journal of Animal Ecology},

volume = {87},

number = {5},

pages = {1331--1341},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{bonnaffe_ontogenetic_2018,

title = {Ontogenetic trajectories of body coloration reveal its function as a multicomponent nonsenescent signal},

author = {Willem Bonnaffé and Mélissa Martin and Marianne Mugabo and Sandrine Meylan and Jean-François Le Galliard},

url = {https://onlinelibrary.wiley.com/doi/full/10.1002/ece3.4369},

doi = {https://doi.org/10.1002/ece3.4369},

year  = {2018},

date = {2018-01-01},

journal = {Ecology and evolution},

volume = {8},

number = {24},

pages = {12299--12307},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{dupoue_shorter_2017,

title = {Shorter telomeres precede population extinction in wild lizards},

author = {Andréaz Dupoué and Alexis Rutschmann and Jean-François Le Galliard and Jean Clobert and Frédéric Angelier and Coline Marciau and Stéphanie Ruault and Donald Miles and Sandrine Meylan},

url = {https://www.nature.com/articles/s41598-017-17323-z},

doi = {10.1038/s41598-017-17323-z},

issn = {2045-2322},

year  = {2017},

date = {2017-12-01},

urldate = {2018-01-15},

journal = {Scientific Reports},

volume = {7},

number = {1},

pages = {16976},

abstract = {Identifying the early warning signals of catastrophic extinctions has recently become a central focus for ecologists, but species’ functional responses to environmental changes remain an untapped source for the sharpening of such warning signals. Telomere length (TL) analysis represents a promising molecular tool with which to raise the alarm regarding early population decline, since telomere attrition is associated with aging processes and accelerates after a recurrent exposure to environmental stressors. In the southern margin of their range, populations of the common lizard (Zootoca vivipara) recently became extinct at lowest elevations due to changes in climate conditions. However, the proximal signals involved in these demographic declines are still unknown. Here, we sampled 100 yearling lizards from 10 natural populations (n = 10 per population) along an extinction risk gradient. Relative lizard abundance dramatically dropped over 12 years in low-altitude populations characterized by warmer ambient temperatures and higher body growth of lizards early in life. A non-linear relationship was found between TL and population extinction risk, with shorter telomeres in populations facing high risk of extinction when compared to non-threatened ones. Our results identify TL as a promising biomarker and imply that population extinctions might be preceded by a loop of physiological aging.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{artacho_geographic_2017,

title = {Geographic variation and acclimation effects on thermoregulation behavior in the widespread lizard Liolaemus pictus},

author = {Paulina Artacho and Samuel Perret and Julia Saravia and Jose Luis Bartheld and Jean-François Le Galliard},

url = {https://www.sciencedirect.com/science/article/abs/pii/S0306456516301097},

doi = {https://doi.org/10.1016/j.jtherbio.2016.11.001},

year  = {2017},

date = {2017-01-01},

journal = {Journal of Thermal Biology},

volume = {63},

pages = {78--87},

abstract = {Populations at the warm range margins of the species distribution may be at the greatest risks of extinction from global warming unless they can tolerate extreme environmental conditions. Yet, some studies suggest that the thermal behavior of some lizard species is evolutionarily rigid. During two successive years, we compared the thermal biology of two populations of Liolaemus pictus living at the northern (warmer) and one population living at the southern (colder) range limits, thus spanning an 800 km latitudinal distance. Populations at the two range margins belong to two deeply divergent evolutionary clades. We quantified field body temperatures (Tb), laboratory preferred body temperatures (PBT), and used operative temperature data (Te) to calculate the 

effectiveness of thermoregulation (E). During one year in all populations, we further exposed half of the lizards to a cold or a hot acclimation treatment to test for plasticity in the thermal behavior. The environment at the southern range limit was characterized by cooler weather and lower Te. Despite that, females had higher Tb and both males and females had higher PBT in the southernmost population (or clade) than in the northernmost populations. Acclimation to cold conditions led to higher PBT in all populations suggesting that plastic responses to thermal conditions, instead of evolutionary history, may contribute to geographic variation. Lizards regulated moderately well their body temperature (E≈0.7): they avoided warm microhabitats in the northern 

range but capitalized on warm microhabitats in the southern range. We review literature data to show that Liolaemus species increase their thermoregulation efficiency in thermally challenging environments. Altogether, this indicates that habitats of low thermal quality generally select against thermoconformity in these lizards.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{dupoue_water_2017,

title = {Water availability and environmental temperature correlate with geographic variation in water balance in common lizards},

author = {Andréaz Dupoué and Alexis Rutschmann and Jean-François Le Galliard and Donald B Miles and Jean Clobert and Dale F DeNardo and George A Brusch and Sandrine Meylan},

url = {https://link.springer.com/article/10.1007/s00442-017-3973-6},

doi = {10.1007/s00442-017-3973-6},

issn = {0029-8549, 1432-1939},

year  = {2017},

date = {2017-01-01},

urldate = {2018-01-04},

journal = {Oecologia},

volume = {185},

number = {4},

pages = {561--571},

abstract = {Water conservation strategies are well documented in species living in water-limited environments, but physiological adaptations to water availability in temperate climate environments are still relatively overlooked. Yet, temperate species are facing more frequent and intense droughts as a result of climate change. Here, we examined variation in field hydration state (plasma osmolality) and standardized evaporative water loss rate (SEWL) of adult male and pregnant female common lizards (Zootoca vivipara) from 13 natural populations with contrasting air temperature, air humidity, and access to water. We found different patterns of geographic variation between sexes. Overall, males were more dehydrated (i.e. higher osmolality) than pregnant females, which likely comes from differences in field behaviour and water intake since the rate of SEWL was similar between sexes. Plasma osmolality and SEWL rate were positively correlated with environmental temperature in males, while plasma osmolality in pregnant females did not correlate with environmental conditions, reproductive stage or reproductive effort. The SEWL rate was significantly lower in populations without access to free standing water, suggesting that lizards can adapt or adjust physiology to cope with habitat dryness. Environmental humidity did not explain variation in water balance. We suggest that geographic variation in water balance physiology and behaviour should be taken account to better understand species range limits and sensitivity to climate change.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{gallardo_ruiz_genetic_2017,

title = {Genetic variation in light vision and light-dependent movement behaviour in the eyeless Collembola Folsomia candida},

author = {Marta Gallardo Ruiz and Jean-François Le Galliard and Thomas Tully},

url = {https://www.sciencedirect.com/science/article/abs/pii/S003140561630138X},

doi = {https://doi.org/10.1016/j.pedobi.2016.12.001},

issn = {0031-4056},

year  = {2017},

date = {2017-01-01},

journal = {Pedobiologia},

volume = {61},

pages = {33--41},

abstract = {Abstract Animals can cope with spatiotemporal variation in their environment through mobility and selective habitat choice. Intra-specific variation in habitat choice has been documented especially for host plant preferences and cryptic habitat selection in insects. Here, we investigated the genetic variation in light sensitivity and light-dependent habitat choice in the eyeless Collembola Folsomia candida with a choice test under four different lighting conditions (control dark condition, two simulations of undergrowth natural light conditions and red light). We tested twelve clonal strains from diverse geographical origins that are clustered in two evolutionary clades with contrasting fast or slow life-history strategies. The clones differed in their mean movement probabilities in the dark treatment. These differences were related to the two different phylogenetic clades, where fast-life history clones are on average more mobile than slow-life history counterparts as predicted by the ‘colonizer syndrome’ hypothesis. We found behavioural avoidance of light in the three light conditions. Moreover, photophobia was stronger when the simulated light spectrum was brighter and included non-red light. Photophobia was similar among all clonal lineages and between the two clades, which suggests that this behaviour is a shared behavioural trait in this species. We discuss the use of light as an environmental cue for orientation, displacement and habitat choice under natural conditions.},

keywords = {dispersal, Folsomia candida, light sensitivity, phenotypic plasticity},

pubstate = {published},

tppubtype = {article}

}

@article{josserand_habitat_2017,

title = {Habitat degradation increases stress-hormone levels during the breeding season and decreases survival and reproduction in adult common lizards. Oecologia. In press},

author = {Rémy Josserand and Andréaz Dupoué and Simon Agostini and Claudy Haussy and Jean-François Le Galliard and Sandrine Meylan},

url = {https://link.springer.com/article/10.1007%2Fs00442-017-3841-4},

doi = {https://doi.org/10.1007/s00442-017-3841-4},

year  = {2017},

date = {2017-01-01},

journal = {Oecologia},

volume = {184},

number = {1},

pages = {75--86},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{mugabo_sex-specific_2017,

title = {Sex-specific density-dependent secretion of glucocorticoids in lizards: insights from laboratory and field experiments},

author = {Marianne Mugabo and Jean-François Le Galliard and Samuel Perret and Beatriz Decencière and Claudy Haussy and Sandrine Meylan},

url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/oik.03701},

doi = {10.1111/oik.03701},

issn = {1600-0706},

year  = {2017},

date = {2017-01-01},

urldate = {2020-04-09},

journal = {Oikos},

volume = {126},

number = {7},

pages = {1051--1061},

abstract = {Negative density feedbacks have been extensively described in animal species and involve both consumptive (i.e. trophic interactions) and non-consumptive (i.e. social interactions) mechanisms. Glucocorticoids are a major component of the physiological stress response and homeostasis, and therefore make a good candidate for proximate determinants of negative density feedbacks. Here, we combined laboratory and field experiments with enclosed populations to investigate the relationship between density, social stress and plasma corticosterone levels in the common lizard Zootoca vivipara. This species exhibits strong negative density feedbacks that affect females more than males, and its life history is sensitive to experimentally-induced chronic elevation of corticosterone plasma levels. We found that prolonged crowding in the laboratory can trigger a chronic secretion of corticosterone independent from food restriction. In the field experiments, corticosterone levels of females were not affected by population density. Corticosterone levels of males increased with population density but only during the late activity season in a first field experiment where we manipulated density. They also increased with density during the mating season but only in populations with a female-biased sex ratio in a second field experiment where we crossed manipulated density and adult sex ratio. Altogether, our results provide limited evidence for a role of basal corticosterone secretion in density feedbacks in this species. Context and density-dependent effects in males may arise from changes in behavior caused by competition for resources, male–male competition, and mating.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{voituron_chronic_2017,

title = {Chronic stress, energy transduction, and free-radical production in a reptile},

author = {Yann Voituron and Rémy Josserand and Jean-François Le Galliard and Claudy Haussy and Damien Roussel and Caroline Romestaing and Sandrine Meylan},

url = {https://link.springer.com/article/10.1007/s00442-017-3933-1},

doi = {https:doi.org10.1007s00442-017-3933-1},

issn = {1432-1939},

year  = {2017},

date = {2017-01-01},

journal = {Oecologia},

volume = {185},

number = {2},

pages = {195--203},

abstract = {Stress hormones, such as corticosterone, play a crucial role in orchestrating physiological reaction patterns shaping adapted responses to stressful environments. Concepts aiming at predicting individual and population responses to environmental stress typically consider that stress hormones and their effects on metabolic rate provide appropriate proxies for the energy budget. However, uncoupling between the biochemical processes of respiration, ATP production, and free-radical production in mitochondria may play a fundamental role in the stress response and associated life histories. In this study, we aim at dissecting sub-cellular mechanisms that link these three processes by investigating both whole-organism metabolism, liver mitochondrial oxidative phosphorylation processes (O2 consumption and ATP production) and ROS emission in Zootoca vivipara individuals exposed 21 days to corticosterone relative to a placebo. Corticosterone enhancement had no effect on mitochondrial activity and efficiency. In parallel, the corticosterone treatment increased liver mass and mitochondrial protein content suggesting a higher liver ATP production. We also found a negative correlation between mitochondrial ROS emission and plasma corticosterone level. These results provide a proximal explanation for enhanced survival after chronic exposure to corticosterone in this species. Importantly, none of these modifications affected resting whole-body metabolic rate. Oxygen consumption, ATP, and ROS emission were thus independently affected in responses to corticosterone increase suggesting that concepts and models aiming at linking environmental stress and individual responses may misestimate energy allocation possibilities.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}